Download e-book for iPad: Amino Acid Analysis Protocols (Methods in Molecular Biology by Cooper C., Packer N.

By Cooper C., Packer N.

ISBN-10: 0896036561

ISBN-13: 9780896036567

ISBN-10: 1592590470

ISBN-13: 9781592590476

Proteome platforms Ltd, North Ryde, Australia. Describes numerous amino acid research thoughts and the way every one approach can be utilized to reply to particular biologic questions.

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Extra resources for Amino Acid Analysis Protocols (Methods in Molecular Biology Vol 159)

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Kivirikko, K. , and Pihlajaniemi, T. (1992) Hydroxylation of proline and lysine residues in collagens and other animal and plant proteins, in Posttranslational Modifications of Proteins (Harding, J. J. and Crabbe, M. ), CRC, Boca Raton, FL, pp. 1–51. 9. Molony, M. , and Harris, R. J. ), Academic, San Diego, CA, pp. 91–98. 30 Macchi et al. 10. Kornfeld, R. and Kornfeld, S. (1985) Assembly of asparagine-linked oligosaccharides. Annu. Rev. Biochem. 54, 631–664. 11. Tarentino, A. , Gomez, C. , and Plummer, T.

Anal. Biochem. 266, 31–47. 17. Jones, M. , Merewether, L. , Clogston, C. , and Lu, H. S. (1994) Peptide map analysis of recombinant human granulocyte stimulating factor: elimination of methionine modification and nonspecific cleavages. Anal. Biochem. 216, 135–146. 18. Allen, G. (1989) Determination of the carboxy-terminal residue, in Sequencing of Proteins and Peptides, Elsevier, Amsterdam and New York, pp. 67–71. 19. Grunau, J. A. and Swaider, J. M. (1992) Chromatography of 99 amino acids and other ninhydrin-reactive compounds in the Pickering lithium gradient system.

2. After removal of the acid, reconstitute the samples with Na-S buffer, and analyze using the standard program (Table 1) (see Note 25). Representative chromato- 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 20 Macchi et al. Fig. 2. Summary sheet using the AAA macro. Average compositions are given in the right-hand column, and the average mg/mL value is provided in the lower right hand box. CyA refers to cysteic acid, which is present when samples are oxidized intentionally. grams for the standard mixture containing carboxymethylcysteine (CMCys) and for an S-carboxymethylated recombinant antibody sample are given in Fig.

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Amino Acid Analysis Protocols (Methods in Molecular Biology Vol 159) by Cooper C., Packer N.


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