Download e-book for kindle: Advances in Botanical Research, Vol. 11 by J.A. Callow, H.W. Woolhouse (Eds.)

By J.A. Callow, H.W. Woolhouse (Eds.)

ISBN-10: 0120059118

ISBN-13: 9780120059119

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Sample text

The ability to measure flow has been demonstrated for both systems; Johnson showed that the velocity derived from light scattering compared well with results from other methods. V. BIOLOGICAL APPLICATIONS A . PARTICLES 1 . Particle Characterization a . In Vitro Studies. Light scattering techniques have achieved considerable success in the estimation of the size and shape of particles from their translational and rotational diffusion coefficients. The techniques are applicable to a very wide range of particle sizes from small proteins to large viruses and bacteria (see Bloomfield, 1981, for a recent review).

Typical sets of results are given in Table 111. , labeled “lambda” by Provencher), is proportional to the diffusion coefficient of the particle, D (Section III,D), and the relative signal level, a,depends upon relative number density of the particle. The individual component values of r exhibit considerable variation (compare different runs in Table III). This variation occurs both within the same pollen tube and between different tubes. There is a more consistent relationship, however, between the r values for the different components within 10 20 1 1 L 30 40 50 TIME (rnsec) I 60 Fig.

This view is further supported by the observation that the signal levels (a2) for r2are relatively higher in correlation functions recorded at the extreme tip, where vesicle numbers are greatest (Fig. 17). If the r values and particle sizes (from electron microscopy) are used to calculate local (intrinsic) viscosity values, it is found that viscosities of 2-72 CP are present (Table 111). These values lie within the broad range already believed to occur in living cells (see Section 11,A). The mitochondria experience higher effective viscosities than the vesicles; however, the ratio between their respective viscosities remains remarkably constant despite changes in their absolute values.

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Advances in Botanical Research, Vol. 11 by J.A. Callow, H.W. Woolhouse (Eds.)

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