By P.G. LeFevre
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Additional resources for Active Transport through Animal Cell Membranes
The turnover in a cat's heart (Fig. 16) is about twenty times as rapid as in its skeletal muscle, possibly by reason of this very response to activity (SACKS 1948). It appears from FURC;HGOTT and SHORR'S (1943) report that this entrance of phosphate into the cardiac muscle by way of formation of organic phosphates is not absolutely essential to its penetration. In dog ventricular slices in labelled-phosphate Ringer, in which phosphorylation was inhibited by cold, no prominent organic P-compound was found to attain a given specific activity prior to the intracellular orthophosphate.
In the absence of glucose. fluoride plus either malonate or azide also proved to be effective inhibitory combinations; but neither of the latter two agents assisted F- in inhibition of glucose oxidation. -MuLLINS (1942) showed that P32-phosphate uptake in yeast was closely associated with carbohydrate metabolism. With higher temperahIres and ample sugar supply, uptake was rapid and was little affected by anoxia. Like K +, the phosphate was later released to the medium as the metabolic activity subsided.
Wu C. . Na+ is here removed from medium, while almost none is lost from particles. "Cyclophorase" preparations all incubated 30 minutes at 20°C. 5 mM. ~aHC03 STANBURY and MUDGE (1953) attempted to analyse the relation of the mitochondrial K+ maintenance to the metabolic activity in similar preparations from rabbit liver. ; upsets in pH or osmotic pressure had to be rather extreme to affect the K+ accumulation, but ultimately caused a depression which paralleled the drop in the oxygen consumption.
Active Transport through Animal Cell Membranes by P.G. LeFevre